Investigation of sequential and enzymatic extraction of arsenic from drinking water distribution solids using ICP-MS

A sequential extraction approach was utilized to estimate the distribution of arsenite [As(iii)] and arsenate [As(v)] on iron oxide/hydroxide solids obtained from drinking water distribution systems. The arsenic (As) associated with these solids can be segregated into three operationally defined categories (exchangeable, amorphous and crystalline) according to the sequential extraction literature. The exchangeable As, for the six drinking water solids evaluated, was estimated using 10 mM MgCl(2) and 10 mM NaH(2)PO(4) and represented between 5-34% of the total As available from the solid. The amorphously bound As was estimated using 10 mM (NH(4))(2)C(2)O(4) and represented between 57-124% of the As available from the respective solid. Finally, the crystalline bound As was estimated using titanium citrate and this represented less than 1.5% of the As associated with the solids. A synthetic stomach/intestine extraction approach was also applied to the distribution solids. The stomach fluid was found to extract between 0.5-33.3 microg g(-1) As and 120-2,360 microg g(-1) iron (Fe). The As concentrations in the intestine fluid were between 0.02-0.04 microg g(-1) while the Fe concentration ranged from 0.06-0.7 microg g(-1) for the first six drinking water distribution solids. The elevated Fe levels associated with the stomach fluid were found to produce Fe based precipitates when the intestinal treatment was applied. Preliminary observations indicate that most of the aqueous Fe in the stomach fluid is ferric ion and the observed precipitate produced in the intestine fluid is consistent with the decreased solubility of ferric ion at the pH associated with the intestine.     

Solar energy harvesting in the epicuticle of the oriental hornet (Vespa orientalis)

The Oriental hornet worker correlates its digging activity with solar insolation. Solar radiation passes through the epicuticle, which exhibits a grating-like structure, and continues to pass through layers of the exo-endocuticle until it is absorbed by the pigment melanin in the brown-colored cuticle or xanthopterin in the yellow-colored cuticle. The correlation between digging activity and the ability of the cuticle to absorb part of the solar radiation implies that the Oriental hornet may harvest parts of the solar radiation. In this study, we explore this intriguing possibility by analyzing the biophysical properties of the cuticle. We use rigorous coupled wave analysis simulations to show that the cuticle surfaces are structured to reduced reflectance and act as diffraction gratings to trap light and increase the amount absorbed in the cuticle. A dye-sensitized solar cell (DSSC) was constructed in order to show the ability of xanthopterin to serve as a light-harvesting molecule.     

A process-based population dynamics model to explore target and non-target impacts of a biological control agent

The risks and benefits associated with efforts to control invasive alien species using classical biological control are being subjected to increasing scrutiny. A process-based population dynamics model was developed to explore the interactions between a folivorous biological control agent, Cleopus japonicus, and its plant host Buddleja davidii. The model revealed that climate could have a significant impact upon the interactions between B. davidii and C. japonicus. At the coolest sites, the impact of C. japonicus on B. davidii was slowed, but it was still eventually capable of controlling populations of B. davidii. At the warmer sites where both B. davidii and C. japonicus grew faster, B. davidii succumbed rapidly to weevil damage. We hypothesise that barring an encounter with a natural enemy, C. japonicus will eventually be able to provide sustained control B. davidii throughout the North Island of New Zealand. The model scenarios illustrate the potential for the C. japonicus population to attain high densities rapidly, and to defoliate patches of B. davidii, creating the potential for spill-over feeding on non-target plants. The potential magnitude of this threat will depend partly on the climate suitability for C. japonicus, the pattern by which it migrates in response to a reduction in the available leaf resource, and the suitability of non-target plants as hosts. In all migration scenarios considered, the pattern of population growth and resource consumption by C. japonicus was exponential, with a strong tendency toward complete utilisation of resource patches more quickly at the warmer compared to colder sites. In addition to providing some useful hypotheses about the effects of climate on the biological control system, and the non-target risks, it also provides some insight into the mechanisms by which climate affects the system.     

Shark personalities? Repeatability of social network traits in a widely distributed predatory fish

Interest in animal personalities has generated a burgeoning literature on repeatability in individual traits such as boldness or exploration through time or across different contexts. Yet, repeatability can be influenced by the interactive social strategies of individuals, for example, consistent inter-individual variation in aggression is well documented. Previous work has largely focused on the social aspects of repeatability in animal behaviour by testing individuals in dyadic pairings. Under natural conditions, individuals interact in a heterogeneous polyadic network. However, the extent to which there is repeatability of social traits at this higher order network level remains unknown. Here, we provide the first empirical evidence of consistent and repeatable animal social networks. Using a model species of shark, a taxonomic group in which repeatability in behaviour has yet to be described, we repeatedly quantified the social networks of ten independent shark groups across different habitats, testing repeatability in individual network position under changing environments. To understand better the mechanisms behind repeatable social behaviour, we also explored the coupling between individual preferences for specific group sizes and social network position. We quantify repeatability in sharks by demonstrating that despite changes in aggregation measured at the group level, the social network position of individuals is consistent across treatments. Group size preferences were found to influence the social network position of individuals in small groups but less so for larger groups suggesting network structure, and thus, repeatability was driven by social preference over aggregation tendency.     

Divergence in sink contributions to population persistence

Population sinks present unique conservation challenges. The loss of individuals in sinks can compromise persistence; but conversely, sinks can improve viability by improving connectivity and facilitating the recolonization of vacant sources. To assess the contribution of sinks to regional population persistence of declining populations, we simulated source–sink dynamics for 3 very different endangered species: Black‐capped Vireos (Vireo atricapilla) at Fort Hood, Texas, Ord's kangaroo rats (Dipodomys ordii) in Alberta, and Northern Spotted Owls (Strix occidentalis caurina) in the northwestern United States. We used empirical data from these case studies to parameterize spatially explicit individual‐based models. We then used the models to quantify population abundance and persistence with and without long‐term sinks. The contributions of sink habitats varied widely. Sinks were detrimental, particularly when they functioned as strong sinks with few emigrants in declining populations (e.g., Alberta's Ord's kangaroo rat) and benign in robust populations (e.g., Black‐capped Vireos) when Brown‐headed Cowbird (Molothrus ater) parasitism was controlled. Sinks, including ecological traps, were also crucial in delaying declines when there were few sources (e.g., in Black‐capped Vireo populations with no Cowbird control). Sink contributions were also nuanced. For example, sinks that supported large, variable populations were subject to greater extinction risk (e.g., Northern Spotted Owls). In each of our case studies, new context‐dependent sinks emerged, underscoring the dynamic nature of sources and sinks and the need for frequent re‐assessment. Our results imply that management actions based on assumptions that sink habitats are generally harmful or helpful risk undermining conservation efforts for declining populations.     

Continuous automated imaging-in-flow cytometry for detection and early warning of Karenia brevis blooms in the Gulf of Mexico

Monitoring programs for harmful algal blooms (HABs) typically rely on time-consuming manual methods for identification and enumeration of phytoplankton, which make it difficult to obtain results with sufficient temporal resolution for early warning. Continuous automated imaging-in-flow by the Imaging FlowCytobot (IFCB) deployed at Port Aransas, TX has provided early warnings of six HAB events. Here we describe the progress in automating this early warning system for blooms of Karenia brevis. In 2009, manual inspection of IFCB images in mid-August 2009 provided early warning for a Karenia bloom that developed in mid-September. Images from 2009 were used to develop an automated classifier that was employed in 2011. Successful implementation of automated file downloading, processing and image classification allowed results to be available within 4 h after collection and to be sent to state agency representatives by email for early warning of HABs. No human illness (neurotoxic shellfish poisoning) has resulted from these events. In contrast to the common assumption that Karenia blooms are near monospecific, post-bloom analysis of the time series revealed that Karenia cells comprised at most 60–75 % of the total microplankton.     

Mercury Measurement and Its Control: What We Know,Have Learned,and Need to Further Investigate

Disposal of mercury waste has always provided unique challenges due to its high degree of complexity and volatility. This study evaluated the feasibility of using waste LF slag to form a cementitious matrix capable of providing an effective stabilization/solidification solution for the treatment of mercury wastes. The new matrix was synthesized and simulated through a combination of alkali activation and autoclaving process and doped with mercury nitrate at increasing dosage while monitoring the final form of the mercury and its effects on the mineral stability and structure of the new matrix. Compressive strength of up to 20 N/mm² was achievable for the original matrix. Promising results were obtained in terms of reduced leachability of the mercury when compared to ordinary Portland cement systems at low doping concentration of around 0.5% by weight. A series of precipitation reactions was found to be the main cause responsible for this successful stabilization, especially the metal sulfide precipitation that occurred with the sulfur present in the original waste LF slag.

Implications: Using waste to treat waste as a concept is a new approach that not only solves waste disposal problems but also minimizes the toxicity and the potential hazard of leaching of heavy metals. This study evaluated the feasibility of using waste ladle furnace slag generated from steel-making industries to form a cementitious matrix capable of providing an effective stabilization/solidification solution for the treatment of mercury-containing wastes. Promising results were obtained, and it clearly showed this approach is feasible, which could be a one-stone-kills-two-birds solution for mercury stabilization as well as an industrial waste disposal problem.     

Projections of 21st Century Sierra Nevada Local Hydrologic Flow Components Using an Ensemble of General Circulation Models1

Abstract: Sierra Nevada snowmelt and runoff is a key source of water for many of California’s 38 million residents and nearly the entire population of western Nevada. The purpose of this study was to assess the impacts of expected 21st Century climatic changes in the Sierra Nevada at the subwatershed scale, for all hydrologic flow components, and for a suite of 16 General Circulation Models (GCMs) with two emission scenarios. The Soil and Water Assessment Tool (SWAT) was calibrated and validated at 35 unimpaired streamflow sites. Results show that temperatures are projected to increase throughout the Sierra Nevada, whereas precipitation projections vary between GCMs. These climatic changes drive a decrease in average annual streamflow and an advance of snowmelt and runoff by several weeks. The largest streamflow reductions were found in the mid‐range elevations due to less snow accumulation, whereas the higher elevation watersheds were more resilient due to colder temperatures. Simulation results showed that decreases in snowmelt affects not only streamflow, but evapotranspiration, surface, and subsurface flows, such that less water is available in spring and summer, thus potentially affecting aquatic and terrestrial ecosystems. Declining spring and summer flows did not equally affect all subwatersheds in the region, and the subwatershed perspective allowed for identification for the most sensitive basins throughout the Sierra Nevada.     

Attribute-based analysis of hunters’ lease preferences

Understanding of hunter preferences for hunting lease attributes is important to landowners because such knowledge provides key information for managing and marketing fee-hunting in order to maximize revenues. Premised on this insight, we used attribute based modeling to investigate how hunter preferences for potential leases were influenced by lease and hunter-specific attributes. A mail survey of Mississippi licensed hunters provided the necessary data. Estimation results based on McFadden conditional logit regression suggested that lease attributes including game diversity, lease location relative to hunter residence, lease size, lease duration and lease rate influenced willingness to pay for additional units of lease attributes. Depending on the specific levels of these attributes, WTP could vary as much as $5.70 per acre. Of the hunter-specific attributes, age and income significantly influenced hunter decision to buy a lease or opt for status quo. Results of this study should assist landowners in increasing financial returns from fee-hunting endeavors through appropriate changes to their hunting access policies and wildlife management activities in response to hunter preferences regarding lease attributes.     

Morphological and molecular variation within an ocean basin in wedge-tailed shearwaters (Puffinus pacificus)

Micro-evolutionary processes that underpin genetic and morphological variation in highly mobile pelagic vertebrates are virtually unknown. Previous findings preferentially invoke vicariant isolation due to large-scale physical barriers such as continental landmasses, followed by genetic drift. However increasingly, evidence for divergence by non-random processes (e.g. selection, plasticity) is being presented. Wedge-tailed shearwaters are wide-ranging seabirds with breeding colonies located such that they experience a variety of environmental pressures and conditions. Previous work on this species has provided evidence of inter-colony divergence of adult morphology and foraging modes, as well as chick developmental patterns, suggesting that reinforcement among colonies is possible. In order to evaluate the micro-evolutionary processes driving this observed variation, our study compared patterns of gene flow with morphological and environmental variation among four colonies of wedge-tailed shearwater breeding within the Indo-Pacific Ocean basin. Estimates of gene flow differed according to the genetic marker used; most likely, this is a function of different mutation rates. Nuclear introns suggest that gene flow among wedge-tailed shearwater breeding colonies within the Indo-Pacific Ocean basin is substantial, however microsatellite markers imply that gene flow is reduced. In general, levels of genetic divergence were relatively low and did not correlate with geographic distance, morphological distance or environmental differences (sea-surface temperature and chlorophyll a concentration) among colonies. We suggest that genetic drift alone is unlikely to be the major source of morphological variation seen in this species. Instead, we propose that non-random processes (selection, plasticity) underpin morphological diversity seen in this and possibly other seabird species.